Insertion junction: CLIP2.029628_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.029628
Chromosome:chromosome 9
Location:4892021
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801061 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACAGCCCATTTCACTCAGAAGCGGACGCAGAACAGCACGCCAGCAAC
Internal bar code:TTACTACTACATCAAAGAGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3831
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TAGAATCGTGCGGCTGCTAG
Suggested primer 2:GCATGCTATTGTGCCTTCCG