| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029650 |
| Chromosome: | chromosome 3 |
| Location: | 1966222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155501 | (1 of 1) PTHR30269 - TRANSMEMBRANE PROTEIN YFCA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACACTAACACATATGCATACGTGCTCTTACGCGCCACTTTGCCCGCA |
| Internal bar code: | TAATGTTGAAAGACTGCCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4275 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTTGACCCGGATCCCTCA |
| Suggested primer 2: | GGTATAGTCCTCGGCCTTGC |