| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.029651 |
| Chromosome: | chromosome 3 |
| Location: | 3005550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g800312 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGGCGAGTCTGTTGTTTTGGGTCAGGAAGTAAAGGGCGGCAGCGACG |
| Internal bar code: | TTCCTACATTTCTCTATCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1315 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCAGTTTTGGTGTGACG |
| Suggested primer 2: | GAAGACAGAGGGAGGGTTGC |