Insertion junction: CLIP2.029671_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.029671
Chromosome:chromosome 1
Location:5528340
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039400 (1 of 1) 3.8.1.5 - Haloalkane dehalogenase / 1-chlorohexane halidohydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGGAAACTTGTCGTCGCTCCCCGGATTCCACCCCGCGCTCCCTCGA
Internal bar code:TGTGGGCGCTGTTGGGTTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:992
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGGCATACGGGCTAATC
Suggested primer 2:GTGCAACGTTTACACACGCT