Insertion junction: CLIP2.029683_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029683
Chromosome:chromosome 10
Location:1737089
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430200 ARS3 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCGCACCTTGCCAATGGAGTAGACGTTGTACCCCGCCTTCTGCAGCTG
Internal bar code:CGTAGCGGTCCCTTGTAGTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1619
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAAACAGGACCAGCTGC
Suggested primer 2:CACAGGCTAGCAGGGATAGC