Insertion junction: CLIP2.029695_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029695
Chromosome:chromosome 11
Location:1686996
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468350 (1 of 1) K00486 - kynurenine 3-monooxygenase (KMO) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACCGTACACAAGCTCACACATACACACATACCTTGGGCTGC
Internal bar code:GATTTACGTCTGTGCCTACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:307
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTTCACTCCGCCTTCT
Suggested primer 2:TGCGAGGGAGGAACAAACTC