Insertion junction: CLIP2.029710_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.029710
Chromosome:chromosome 16
Location:4764890
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g687850 PTK22 (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype; Protein of unknown function CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGGCGCTTGAGTCGCTGAGCCTGCGCATGTGTGAGGTGACCAACCT
Internal bar code:GATATTTTTTTAAAGCTTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1960
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCATCCGCATCATCACG
Suggested primer 2:CGGCATTCGCATTAACAGGG