| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029716 |
| Chromosome: | chromosome 12 |
| Location: | 4195863 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517900 | SCA1,SECA1 | (1 of 1) K03070 - preprotein translocase subunit SecA (secA); Chloroplast-associated SecA protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGCGGTTTGTTGAGGTGTACTATGTACCGCCTGGCCCAGGCCGCTG |
| Internal bar code: | CATGTCGACATAGTGGCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2403 |
| LEAP-Seq percent confirming: | 63.3333 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCCTTCGGCACATTT |
| Suggested primer 2: | ATGGCGGTGTTAAGATGGGG |