Insertion junction: CLIP2.029722_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029722
Chromosome:chromosome 12
Location:9549688
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g540050 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACACACGTCCACACACACACACGTGTCATTCCCCTTAACAGGACCAGC
Internal bar code:CGGTGCGTAGTTTCACGGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:686
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATACACACACACCTCC
Suggested primer 2:CTGAACGTCTCGTCCTGAGG