| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029726 |
| Chromosome: | chromosome 11 |
| Location: | 196950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467557 | (1 of 2) PTHR31389:SF4 - PROTEIN F32B4.1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACCTCGCAGCTGTGACGCGGCCATGTAGGACGTAGGGCATAGTAGTA |
| Internal bar code: | GTTAGTTCACGACAGTTGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1810 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTTGCTATGACAACCC |
| Suggested primer 2: | ACATCCGTAAACCGTCGCTT |