Insertion junction: CLIP2.029735_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.029735
Chromosome:chromosome 6
Location:8691154
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g310200 DGAT3 Diacylglycerol acyltransferase, Type 3; (1 of 1) PTHR11780//PTHR11780:SF8 - NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1 // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGGGGTGCGGCCCGGTTTACGTTCTGCTCGCGTCACTGTGCGTCCACA
Internal bar code:ACCTAGTGCAATAAGATCTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1692
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCGCCTGGGAAGTTGAC
Suggested primer 2:CCGTGCACCAATCAAACGTT