| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029743 |
| Chromosome: | chromosome 6 |
| Location: | 8860799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311600 | RFK3 | Riboflavin kinase; (1 of 1) PTHR22749//PTHR22749:SF1 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAATGGGCCACGCCTCAACACAAAATTCGTCCGCGGAGGAATGCATA |
| Internal bar code: | ACGAGTTGCTAAGGTTAAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3615 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGAGAGGGTCTGAGTCCG |
| Suggested primer 2: | AGGGCCAGGATCTGAAGGAT |