| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029811 |
| Chromosome: | chromosome 1 |
| Location: | 5696202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00249475 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAACAAGTCGTGATGGCCAATCACGCTGCTCGGTCCCAAAACTGCCGC |
| Internal bar code: | CTTGTTAAAGTGAACGTAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3333 |
| LEAP-Seq percent confirming: | 73.913 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGTGGTCACCAGCT |
| Suggested primer 2: | TTGAGCTGCTCCTTCAGGTG |