Insertion junction: CLIP2.029818_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029818
Chromosome:chromosome 3
Location:2428563
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g158800 (1 of 1) 3.1.2.12 - S-formylglutathione hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTCAACCGCCGCCTCGCCCGCCGCCTCGCCTCAAACACAGGCGTGG
Internal bar code:TGAGCTAAGAAAGGTGGTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4955
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGCCAACAAAAGCTCAA
Suggested primer 2:CGCCGTCAATAAGCAGTTCG