Insertion junction: CLIP2.029835_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.029835
Chromosome:chromosome 16
Location:3052907
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g664250 OASTL2,ASL2 O-acetylserine (Thiol)-lyase/cysteine synthase 2; (1 of 1) 4.2.1.22 - Cystathionine beta-synthase / Serine sulfhydrase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCATGCATGGCCCCATTAGGTCCTCCCCATCCTTGTACTCCACCAAAA
Internal bar code:GTGCACAGTTTGAGGATAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:6413
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGTTGCTCTTTCGCTGC
Suggested primer 2:CTTACCGCTTGTGGTGACCT