| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.029842 |
| Chromosome: | chromosome 3 |
| Location: | 709741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145867 | mL113,OPR130,MRPL113 | mitochondrial ribosome protein ml113, Chlamydomonas-specific | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGCGGCGGCCGCCGCCGCCGCTGCCGTCACTGACCCAGATGACC |
| Internal bar code: | TCTATGGCACGAGGTAGCCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2796 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACTGCCATTCACTCTGT |
| Suggested primer 2: | TGACCGGGGGACTAACGTAT |