| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029842 |
| Chromosome: | chromosome 3 |
| Location: | 709747 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145867 | mL113,OPR130,MRPL113 | mitochondrial ribosome protein ml113, Chlamydomonas-specific | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGACCGCCAGTGAGGCGCCGGCGGTGGCGGAGCCGCCGTCCACGGC |
| Internal bar code: | CTCGAGAATATTCTTATGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1956 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCGGGGGACTAACGTAT |
| Suggested primer 2: | CGGACTGCCATTCACTCTGT |