| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029856 |
| Chromosome: | chromosome 1 |
| Location: | 2959555 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018550 | CYG69 | (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGACCGGGAGAAGTGCGTGCTGGCGCCCTGCTCGCCCGCCAACTGC |
| Internal bar code: | ATCGGATCGCTTTACCGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4018 |
| LEAP-Seq percent confirming: | 98.75 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGGTAGGCTCCCACAAC |
| Suggested primer 2: | GACCATGCACTGTGTCTGGA |