| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.029893 |
| Chromosome: | chromosome 8 |
| Location: | 3040030 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375200 | (1 of 1) K07870 - Ras homolog gene family, member T1 (RHOT1, ARHT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAGTATTCGCGCCAATTCCTATCAGAAGTACTAAGAGGATGTAATTA |
| Internal bar code: | TCGACTCGCATACGTAACAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGGCTACAACGACCAG |
| Suggested primer 2: | CATGAGAAGGGGGTGGCAAT |