Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.029901 |
Chromosome: | chromosome 17 |
Location: | 3585469 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTGTGCTCGGCTAGGACCTGGAAGTCCTCATTGACCATTGGAACGCGA |
Internal bar code: | ACTGAGGCATATGGGTCCGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1637 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 7 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATCCCGTTCACACAGCACC |
Suggested primer 2: | GTACGGACTGCGCTCTCTAC |