| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.029901 |
| Chromosome: | chromosome 17 |
| Location: | 3585471 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725100 | (1 of 1) PTHR11390:SF20 - DNA TOPOISOMERASE 3-BETA-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGAAGCTTGCCGATTCGCGCACAAAGCATGACGCCCAGCATTGGCCG |
| Internal bar code: | ACTGAGGCATATGGGTCCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1508 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGACTGCGCTCTCTAC |
| Suggested primer 2: | TATCCCGTTCACACAGCACC |