| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.029916 |
| Chromosome: | chromosome 16 |
| Location: | 6861217 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671700 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCGCTGCTGGGCGGCCTGCCGCCGGTGGTGGAGGACCGCGGCGCCG |
| Internal bar code: | AGCGCGCAGTTAGCAATGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2248 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGAGGAGCATAACGAGC |
| Suggested primer 2: | CATGCGCAACTTCTCCAACC |