Insertion junction: CLIP2.029976_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029976
Chromosome:chromosome 1
Location:1412178
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g007500 NSS1 (1 of 2) PTHR11819//PTHR11819:SF12 - SODIUM/SOLUTE SYMPORTER // HIGH-AFFINITY CHOLINE TRANSPORTER 1; Sodium:solute symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGTCCGGGTTCCACACGCCCGCCTTGTCCGTGTACTGCGGCGGGCTC
Internal bar code:TGCTTAAATTAAGCACGAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAATATCCGCTGGGTCTCCC
Suggested primer 2:AAGGGGATGTAGCTGTGTGC