| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.029979 |
| Chromosome: | chromosome 4 |
| Location: | 2548779 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222500 | BICD1 | Bicaudal D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCTGGAGTGGTGCGTTCGCCCGCCTGCACACATTTGGCCGCGCTAGT |
| Internal bar code: | TGGGGCAGTGGAAGTAATTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACACCTGCTTCTCCAG |
| Suggested primer 2: | CTGACAGTTCAAAGGGGCCT |