| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.029980 |
| Chromosome: | chromosome 14 |
| Location: | 558266 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611552 | TGL22 | (1 of 32) 3.1.1.3 - Triacylglycerol lipase / Triglyceride lipase; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGCAGGGTCGCGCAATGAGGGCGCTGACAAGGCACCAGCGCTGCTTC |
| Internal bar code: | GTATCATGCTGTTAGTTACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1161 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATCGTTGCTTTGTTGG |
| Suggested primer 2: | GACTGTGTGTGAGGGAGGTG |