| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.029983 |
| Chromosome: | chromosome 14 |
| Location: | 624896 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612000 | (1 of 1) K17545 - serine/threonine-protein kinase ULK4 [EC:2.7.11.1] (ULK4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAATGCAACATGGCAACTAAGGCGTGCGCAAAGACAGGCAATGTTGG |
| Internal bar code: | CTTGAGTTCGGCTAGGGTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4261 |
| LEAP-Seq percent confirming: | 95.3846 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGCGTGGTGTATGAAA |
| Suggested primer 2: | CGGATGTTGTGCACGTTGTT |