| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.030000 |
| Chromosome: | chromosome 14 |
| Location: | 1849436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619800 | SPO11B,TOP4 | meoisis-related DNA topoisomerase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCCCGGAGGAGCAGCGGGCGCTCTTCCAGGTGCGGCCGTCTCGCGCCC |
| Internal bar code: | TCTGTGACTAAGAATGAACCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGACGGTGATACGAGG |
| Suggested primer 2: | ACAGGGAAAACGGTCTCGAC |