Insertion junction: CLIP2.030005_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030005
Chromosome:chromosome 2
Location:2721731
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGACGACCCCAGAAGTCAGCTACGACGGCAAGTCCAATGCTCAGCA
Internal bar code:GGGGTCTCGTATGCTCGAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3654
LEAP-Seq percent confirming:98.7013
LEAP-Seq n confirming:76
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCTTGCAAGGGTTACTA
Suggested primer 2:TAAGCGGGGTATCTGGGTCA