| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030023 |
| Chromosome: | chromosome 10 |
| Location: | 4843318 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453400 | MSC4,MSCL1 | (1 of 1) PF00924//PF13202 - Mechanosensitive ion channel (MS_channel) // EF hand (EF-hand_5); Predicted protein with mechanosensitive ion channel domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTGCTCGAAGAACAGGAACAACACCGACTCGAACGCGTTCCTGTTT |
| Internal bar code: | GGGGGGCTAGATCTCCATTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 6.12245 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTTTGCGCTTTGCCTT |
| Suggested primer 2: | CCAAGCAAGACCAAAGGTGC |