Insertion junction: CLIP2.030029_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030029
Chromosome:chromosome 11
Location:3229879
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g476150 Glutamine cyclotransferase; (1 of 1) 2.3.2.5 - Glutaminyl-peptide cyclotransferase / Glutaminyl-tRNA cyclotransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGACCTGCAAGTGCAAAACGGTTAATGGGAGTCCTGGCGCCTCAACGCC
Internal bar code:CCCGCTCTACCGAGTGCGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3259
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:98
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:98

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGAATGGTTGTGTCGCGA
Suggested primer 2:AATGGGGTGGCAGAATGAGG