| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030033 |
| Chromosome: | chromosome 12 |
| Location: | 1455039 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488250 | (1 of 2) K17822 - DCN1-like protein 1/2 (DCUN1D1_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTGAACGGGACTAGCTTGCGCATACTGGGTTTCCTGTGGAGAACTGT |
| Internal bar code: | TATGAAACTGGCTACTCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3513 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATTGCATGCGGTGATG |
| Suggested primer 2: | CTTGCTGGGTATTGAGGGCA |