| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030038 |
| Chromosome: | chromosome 3 |
| Location: | 5636608 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186150 | (1 of 1) PF07047 - Optic atrophy 3 protein (OPA3) (OPA3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGCGCCACCGGATGGTTCAGGGCCCACTTCTCAAACCGGGCGCCCAG |
| Internal bar code: | GAACGTACGGCACGGGAGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1832 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCTCCATTCAATCGGCG |
| Suggested primer 2: | GCTCCAGGTTCTCAACACGA |