Insertion junction: CLIP2.030040_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030040
Chromosome:chromosome 17
Location:6841975
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g747247 FAP2 TPR-domain Flagellar Associated Protein 2; (1 of 1) IPR002151//IPR003593//IPR013026//IPR019734//IPR027417 - Kinesin light chain // AAA+ ATPase domain // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCTCTTTACACCACATGACTACACACACGTGCATACACATGCCCTCA
Internal bar code:ACATTTTGATAGCGTCAATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1181
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTAGAGACGGTGC
Suggested primer 2:CAGAGAGCACAAAGGGAGGG