Insertion junction: CLIP2.030057_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.030057
Chromosome:chromosome 8
Location:695532
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTGTCCGCAGGAATGACATGAGGGGAAGCCCAACGAAGGCCGTCGCCC
Internal bar code:TCCACCAAAGGGTACCGTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:5494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:149
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:149

Suggested primers for confirmation by PCR

Suggested primer 1:CTGATCGCCTCGTAAGGACC
Suggested primer 2:AACAGGCGTGCAAATGTGAC