| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030099 |
| Chromosome: | chromosome 3 |
| Location: | 943333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146707 | (1 of 2) PF02099 - Josephin (Josephin) | CDS | |
| Cre03.g146727 | GOX9 | Glyoxal oxidase 9; (1 of 2) IPR009880//IPR011043//IPR013783//IPR014756//IPR015202//IPR015916 - Glyoxal oxidase, N-terminal // Galactose oxidase/kelch, beta-propeller // Immunoglobulin-like fold // Immunoglobulin E-set // Domain of unknown function DUF1929 // Galactose oxidase, beta-propeller | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCACAGGTGTGCTTTGCAAACCGACTACATGTTGATTGAACATCACG |
| Internal bar code: | CCGAGGTTTGGTAGCGCCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1482 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACATCCTGTCCATCCC |
| Suggested primer 2: | TACGCTTACCTTGCACTGCA |