| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030101 |
| Chromosome: | chromosome 12 |
| Location: | 4014268 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516000 | (1 of 2) PF07978 - NIPSNAP (NIPSNAP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCGCACAGCTCGTACGCCTTGGACTTGGCATCCTGCCACTCCGCCGC |
| Internal bar code: | AGCGTTAGTCCGCAGGTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5299 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATCCACCCCCAGGCATG |
| Suggested primer 2: | CGCTACTGAATTGCACGTCG |