Insertion junction: CLIP2.030136_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.030136
Chromosome:chromosome 10
Location:499958
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421050 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCACATTGTCACTGGGCTGCCCGCGGCACAGCTGCAGCCGCAGCTCCT
Internal bar code:GGTCTCTACACGGAGAACACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2160
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GAATTACCTTGGCCACACGC
Suggested primer 2:CGGATCACTCACCCCATAGC