| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030151 |
| Chromosome: | chromosome 1 |
| Location: | 4897670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034100 | (1 of 2) K01408 - insulysin (IDE, ide) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCTACGAGCGAATAAGAAAACAACAGAGCACAGGGCGGCAGCAGGG |
| Internal bar code: | TGATCGTAGTCGTTTTATAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2229 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGTACAGGTACAGCA |
| Suggested primer 2: | GACACACGTGGAGAAGTGGT |