| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.030165 |
| Chromosome: | chromosome 6 |
| Location: | 4205450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278243 | (1 of 1) PTHR11748//PTHR11748:SF84 - D-LACTATE DEHYDROGENASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGACGCCGCCGCCCTGCCCGGAGCCTTCTGGGCCCTGCGCGGCGCCG |
| Internal bar code: | GAGAAAGTCGTGCGAGATACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGTGGCTGCTTTTGTA |
| Suggested primer 2: | GCACCTTCCTTCACTCCCTC |