| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.030165 |
| Chromosome: | chromosome 6 |
| Location: | 4205452 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278243 | (1 of 1) PTHR11748//PTHR11748:SF84 - D-LACTATE DEHYDROGENASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGCCGCCCGCGCTGCAGCTCCACGAAACTGTTGATGGGGAGGCGGG |
| Internal bar code: | GAGAAAGTCGTGCGAGATACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCTTCCTTCACTCCCTC |
| Suggested primer 2: | TGGTGGTGGCTGCTTTTGTA |