| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030179 |
| Chromosome: | chromosome 10 |
| Location: | 5311714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456600 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTGCAAATCTTTGACTCGATGGGGCCTTGGTCATTACGCCGGGCAGG |
| Internal bar code: | CGTGGATTGTGGTGTCATGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1862 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCCTAGATGAGCGTGGC |
| Suggested primer 2: | CATGTGACTACCTGCCTGCA |