Insertion junction: CLIP2.030198_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.030198
Chromosome:chromosome 4
Location:3723925
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g229250 (1 of 1) PTHR10209//PTHR10209:SF100 - OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCTGCGACGCTCCGTCAGCCGCCGCCGCACCGCCGCCAGCGCCTCCC
Internal bar code:TCCGCGATCTTCTAGCCGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1825
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GGAATGGGATTGGGAAGGGG
Suggested primer 2:TGGCATTTGTCGGTACTGCT