Insertion junction: CLIP2.030212_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.030212
Chromosome:chromosome 16
Location:4273267
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g669400 SMM49 (1 of 14) PF10294 - Lysine methyltransferase (Methyltransf_16); S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTTGACTGGTGAGCGCTGTGTTTGTGGTTCGCGGGAGTGGGGCTGTG
Internal bar code:CATTCAGATAAGTAAAAGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:996
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGATGGAGGTGCTAGTT
Suggested primer 2:CGTAGAAAACCGCTCGCATG