Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | + |
Strain: | CLIP2.030227 |
Chromosome: | chromosome 2 |
Location: | 6127834 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g119700 | (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCAGCGGCGACAGCCGCGGCAGGGGCTGGGTCAGGTGTCGGCTGTG |
Internal bar code: | CTCAATCTGGAATACTGTCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2156 |
LEAP-Seq percent confirming: | 95.2381 |
LEAP-Seq n confirming: | 40 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGTAAGCAATTCCGACGCG |
Suggested primer 2: | CGCATCCCCTAGCTAGGTTG |