Insertion junction: CLIP2.030270_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030270
Chromosome:chromosome 16
Location:1337564
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g651750 TSI,TSI2 isoleucyl-tRNA synthetase; (1 of 2) K01870 - isoleucyl-tRNA synthetase (IARS, ileS) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGAAGAGGGGGTGCGGGGCTGGGCGAGGGAGAAGGAAGTGGGAGTAGA
Internal bar code:GTCAGCCGAATAGTCACATACG

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTCCGAGGCAATGACCAC
Suggested primer 2:TAATCAAGTCCAAGCGGCGT