| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.030281 |
| Chromosome: | chromosome 4 |
| Location: | 3485412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227900 | PF20 | WD-repeat protein of flagellum central pair; (1 of 6) IPR001680//IPR015943//IPR017986//IPR020472 - WD40 repeat // WD40/YVTN repeat-like-containing domain // WD40-repeat-containing domain // G-protein beta WD-40 repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGAGGGTCCTAGTGGGAGGTGGCTATGCGCTGGTAGGCTCCGTGGC |
| Internal bar code: | GGTGGTAATATAGTGAGTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCTTCCTCCCTGCTC |
| Suggested primer 2: | TGATGACTTCCTGCGCAACT |