| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.030294 |
| Chromosome: | chromosome 12 |
| Location: | 5632848 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531500 | DRC3,FAP134 | (1 of 8) PF14580 - Leucine-rich repeat (LRR_9); Nexin-dynein regulatory complex 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGTTGTTGAAGAGCGACAAGTCGACCAGCTTTGTGAGCGTCTCCAGG |
| Internal bar code: | GTCTACGGATCTACAGTATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5433 |
| LEAP-Seq percent confirming: | 97.9167 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAATCGCGGAGGTGGAG |
| Suggested primer 2: | AGAACCAGTCAGCAGTCTGC |