Insertion junction: CLIP2.030300_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.030300
Chromosome:chromosome 12
Location:7351339
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g557850 (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGATGATAGAGCAGCAGCAGCTGCGCCGCCGTCAGCGCGCGGCGCAGTC
Internal bar code:GGTCTAGTAGGACTTACAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1780
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCCCATTCCGTAAAT
Suggested primer 2:GGTTGCTCACAGGTGGCTAT