| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030305 |
| Chromosome: | chromosome 14 |
| Location: | 2956487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801644 | (1 of 1) K02434 - aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit B (gatB, PET112) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGCCATCAGCACCACATGCCGCCAGTCCGTCACCCTTTAACAGCAG |
| Internal bar code: | TGATTCTTACTGTTCCAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTGGAGGTTGCAAGGTT |
| Suggested primer 2: | GGCGTCATGAAGAGGTGTGA |