| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.030315 |
| Chromosome: | chromosome 1 |
| Location: | 5429500 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038300 | (1 of 10) PF02536 - mTERF (mTERF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAGTCAGCGGAGGTGGCCCGCACCACCTCCTCCACGGCCGCATGCT |
| Internal bar code: | CTGTGGGGGGTTTCTGAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCCGAACTGATACGAGC |
| Suggested primer 2: | AGGACAGGAGGTGGCATACT |